Extended Data Fig. 3: Identification of wax primary (1-) alcohol synthesis gene SOH1 and SOH1-LIKE.
a, The 1-alcohol coverage on leaves of soh1-2 mutant. Wax coverage is expressed as microgram per square decimeter leaf surface area. These results were independently replicated at least twice, yielding similar outcomes each time. Data are shown as mean ± s.d., n = 3 of biological replicates. ***P < 0.001 (Student’s two-tailed t-test). Exact P values are listed in the source data. b, Phylogenetic tree of SOH1 paralogues from Arabidopsis. Phylogenetic tree was generated by MEGA using the neighbor-joining method. SOH1 and SOH1-LIKE were highlighted in red. c, The 1-alcohol coverage on leaves of different SOH1-LIKE overexpression lines. Wax coverage is expressed as microgram per square decimeter leaf surface area. These results were independently replicated at least twice, yielding similar outcomes each time. Data are shown as mean ± s.d., n = 3 of biological replicates. **P < 0.01; ***P < 0.001 (Student’s two-tailed t-test). Exact P values are listed in the source data. d, The 1-alcohol coverage on leaves of Col-0, cer4-4, soh1-1 soh1-like and cer4-4 soh1-1 soh1-like mutants. Wax coverage is expressed as microgram per square decimeter leaf surface area. These results were independently replicated at least twice, yielding similar outcomes each time. Data are shown as mean ± s.d., n = 3 of biological replicates. ND, not detectable. **P < 0.01; ***P < 0.001 (Student’s two-tailed t-test). Exact P values are listed in the source data. e, The 1-alcohol coverage on stems of Col-0, soh1-1, soh1-like and soh1-1 soh1-like mutants. Wax coverage is expressed as microgram per square decimeter stem surface area. These results were independently replicated at least twice, yielding similar outcomes each time. Data are shown as mean ± s.d., n = 5 of biological replicates. Not significant (Student’s two-tailed t-test). Exact P values are listed in the source data.
