Extended Data Fig. 5: BTSa and BTSb play a similar role in regulating nodulation.
a and b, Nodulation phenotype (a) and nodule number (b) of hairy roots expressing BTSa or BTSb at 21 DAI. Scar bars = 2 cm. The numbers in the column of (b) represent the number of samples, data are the means ± SD and significant differences were determined by one-way ANOVA (P < 0.05). c, BTSa-MYC and BTSb-MYC expression in hairy roots in (a). Data are the means ± SD of n = 12. d, Characterization of BTSa editing in btsa-1 mutant. The red stars indicate deletion base pairs. e and f, Nodulation phenotypes (e) and nodule number (f) at 21 DAI. Scar bars = 2 cm for roots and 5 mm for nodules. Data are the means ± SD of n = 10, 11, 15 for WT, btsa-1, btsa btsb-2. g, Nitrogenase activity at 21 DAI. Data are the means ± SD of n = 9, 8, 8 for WT, btsa-1, btsa btsb-2. h, Characterization of BTSb editing in btsb-1 mutant. The red stars indicate the deletion base pairs. i and j, Nodulation phenotypes (i) and nodule number (j) at 21 DAI. Scar bars = 2 cm for roots and 5 mm for nodules. Data are the means ± SD of n = 16, 20, 13 for WT, btsa-1, btsa btsb-2. k, Nitrogenase activity of WT, btsb-1 and btsa btsb-2 at 21 DAI. Data are the means ± SD of n = 7, 8, 8 for WT, btsa-1, btsa btsb-2. f, g, j and k, Significant differences were determined by two-sided Student’s t-test (** P < 0.01, ***P < 0.001, ****P < 0.0001). For the box-plot in (f, g, j and k), whiskers indicate minimum and maximum values, line indicates the median, the box boundaries indicate the upper (25th percentile) and lower (75th percentile) quartiles. The experiments were performed for three times with similar results.
