Extended Data Fig. 2: Detection of native NSP1 by anti-NSP1 antibody and CRISPR–Cas9 gene editing of NSP1a. | Nature Plants

Extended Data Fig. 2: Detection of native NSP1 by anti-NSP1 antibody and CRISPR–Cas9 gene editing of NSP1a.

From: The BRUTUS iron sensor and E3 ligase facilitates soybean root nodulation by monoubiquitination of NSP1

Extended Data Fig. 2

a, Western blot of native NSP1a abundance in nodules of WT and nsp1a-1 mutant using anti-NSP1 antibody. The arrow indicates the specific band of NSP1a and NSP1b. The experiment was repeated for three times with similar results. b, Schematic of the NSP1a gene. The nsp1a-1 mutant in the Wm82 background has a 10-bp (540–549 bp) deletion in the coding region induced by CRISPR–Cas9. The red font in the mutant represents the new translational stop codon due to a frame shift arising from Cas9 nuclease activity. c, Diagram of wild-type and mutant NSP1a protein. d, Sanger sequencing chromatograms of wild-type Wm82 and nsp1a-1 mutant. The blue box indicates deleted region in the nsp1a-1 mutant.

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