Extended Data Fig. 5: Autophagy adaptor CFS1 is not recruited to the tonoplast upon cell wall damage.

(a) Confocal micrographs obtained from GFP-ATG8A mCh-CFS1 stably co-expressing Arabidopsis thaliana root cells under mock, Torin (1.5 hours, 9 µM), and ES20-1 (8 hours, 100 µM) treatments. The panel sequence includes single optical slices for GFP-ATG8A and mCh-CFS1, an additional panel for mCh-CFS1 with oversaturation to enhance visualization, a merge of both channels, corresponding bright field images and insets to enhance visualization. Scale bar, 10 µm. (b) Quantification of CFS1 puncta across the different treatment conditions used in (A). One-sided Wilcoxon test compared treatments (n = 10) to mock; significant differences (p < 0.01) are indicated as asterisks. The central line indicates the median, and the upper and lower bounds represent quartile 3 (75th percentile) and quartile 1 (25th percentile), respectively. The whiskers denote the minima and maxima of the data points.