Extended Data Fig. 6: Tonoplast ATG8ylation does not require FERONIA.

(a) Confocal micrographs of GFP-ATG8A expressed in Arabidopsis thaliana lrx3/4/5 triple mutant background (a) or fer4 mutant background (b), treated with mock, Torin (1.5 hours, 9 µM), and ES20-1 (8 hours, 100 µM). The set includes a single optical slice, a maximum intensity projection, and a bright field image for each treatment condition. Representative image from 10 seedlings analyzed under each treatment. Scale bar, 10 µm. (c) Representative images and quantification of vacuolar morphology of late meristematic atrichoblast cells of GFP-ATG8A, GFP-ATG8A fer-4 and GFP-ATG8A lrx3/4/5. MDY‐64 (yellow) staining depicts vacuolar membrane. One-sided Wilcoxon test compared treatments (n = 10) to GFP-ATG8A line; significant differences (p < 0.01) are indicated as asterisks. Scale bar, 10 µm. The central line indicates the median, and the upper and lower bounds represent quartile 3 (75th percentile) and quartile 1 (25th percentile), respectively. The whiskers denote the minima and maxima of the data points. Mean values are represented with ‘x’.