Extended Data Fig. 9: Key ESCRT proteins are not recruited to the tonoplast upon cell wall damage. | Nature Plants

Extended Data Fig. 9: Key ESCRT proteins are not recruited to the tonoplast upon cell wall damage.

From: ATG8ylation of vacuolar membrane protects plants against cell wall damage

Extended Data Fig. 9

Confocal micrographs depicting the localization of ESCRT (Endosomal Sorting Complex Required for Transport) machinery components GFP-FREE1, GFP-ALIX, and VPS23-RFP in Arabidopsis thaliana root cells, under mock, Torin (1.5 hours, 9 µM), and ES20-1 (8 hours, 100 µM) treatments. Each set comprises a single optical slice, a maximum intensity projection, and a corresponding bright field image, facilitating the comparative analysis of ESCRT component dynamics under torin and ES20-1 treatments. Representative image from 10 seedlings analyzed under each treatment. Scale bar, 10 µm.

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