Extended Data Fig. 6: Ion selectivity of CNGC5 and CNGC1.

a, Sequence alignment of ion selectivity filter motifs in 20 Arabidopsis CNGCs. b-d, Density map at the selectivity filter of CNGC1^Ca and CNGC5^Ca. Both samples were prepared with a supplementation of 2 mM Ca²⁺. Selectivity filter regions of two diagonally opposed subunits are shown in sticks, the Ca²⁺ ions along the ion pathway are in green spheres and water molecules are in red spheres. b, The density at the selectivity filter of CNGC1^Ca. Density map is obtained after C4 symmetry processing (contoured at 5.5 σ). c, The density at the selectivity filter of CNGC5^Ca. Density map is obtained after C4 symmetry processing (contoured at 5.5 σ). d, The density at the selectivity filter of CNGC5^Ca. Density map is obtained after C1 symmetry processing (contoured at 4.0 σ). Chain A, C and chain B, D exhibit distinct conformations due to their mobility or asymmetry, and are shown separately.