Extended Data Fig. 9: Representative fluorescent of CNGC5 wild-type, mutants and GFP in HEK 293T cells. | Nature Plants

Extended Data Fig. 9: Representative fluorescent of CNGC5 wild-type, mutants and GFP in HEK 293T cells.

From: Cryo-EM structures of Arabidopsis CNGC1 and CNGC5 reveal molecular mechanisms underlying gating and calcium selectivity

Extended Data Fig. 9

GFP tag was fused to the N terminus of CNGC5. GFP fluorescence was readily detected in cells expressed CNGC5 wild type (WT) and mutants with similar patterns. Independent experiments have been repeated at least three times with similar results. The varying lengths of the gray bars corresponded to 10 µm in all the indicated variant images.

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