Extended Data Fig. 2: Cryo-EM analysis of CNGC5Ca.

a, Gel filtration profile and Coomassie-blue-stained SDS-PAGE analysis of CNGC5 in buffer containing 0.01% GDN. Independent experiments have been repeated at least three times with similar results. b, Representative micrograph of cryo-EM. c, 2D class averages. d, cryo-EM data analysis pipeline. e, Local resolution estimation and gold-standard Fourier shell correlation (FSC) curves. The color represents the local resolution in Ã….