Extended Data Fig. 4: The removal of DNA methylation by SDG2cd-ZF is blocked in the rdd mutant but not in the idm1 mutant, and this effect is not due to the inhibition of protein expression.
From: Histone H3 lysine 4 methylation recruits DNA demethylases to enforce gene expression in Arabidopsis
a-b The western blotting results showing the protein expression levels of SDG2cd-ZF in the rdd mutant and Col-0 background. The ponceau staining indicates the loading control for each sample. c, Scatterplots depicting the correlation of DNA methylation levels and Log2 H3K4me3 ChIP-seq signals in SDG2cd-ZF transgenic lines in the rdd mutant background versus rdd mutant at ZF off-target sites (n = 6,091). Statistical tests were two-sided, and p-values were reported without adjustment for multiple comparisons. d, Histogram depicting the relative McrBc-qPCR values of FWA in Col-0, fwa, idm1 mutant, and T1 transgenic lines of SDG2cd-ZF in the idm1 mutant background (n = 12 biological replicates), respectively. A lower value indicates a relatively higher level of DNA methylation. Data are presented as mean values ± s.e.m.
