Fig. 6: Co-targeting of TRBIP1 and MQ1v resulted in synergistic DNA methylation establishment.
From: Histone H3 lysine 4 methylation recruits DNA demethylases to enforce gene expression in Arabidopsis
a, qRT–PCR assay indicating the relative mRNA level of FWA in fwa and four T2 transgenic lines of TRBIP1–ZF (n = 4 biological replicates). b, Genome browser view showing H3K4me3 ChIP-seq signals over the FWA region in the fwa and TRBIP1–ZF transgenic lines, as well as Myc ChIP-seq signals at FWA in the Col-0, and transgenic lines of Myc–JMJ14 and Myc–JMJ14xTRBIP1–ZF. FLAG–ZF ChIP-seq signal indicates the ZF binding site. c, BS-PCR-seq measuring the CG, CHG and CHH DNA methylation levels of FWA promoter regions in fwa, Col-0 and transgenic lines of TRBIP1–ZF. d, qRT–PCR assay indicating the relative mRNA levels of FWA in fwa rdr6, Col-0 and T1 transgenic lines of dCas9-MQ1v, TRBIP1-dCas9-MQ1v (n = 12 biological replicates). e, Relative McrBC-qPCR values at FWA in fwa rdr6, Col-0 and T1 transgenic lines of dCas9-MQ1v, TRBIP1-dCas9-MQ1v. A lower value indicates a relatively higher level of DNA methylation (n = 12 biological replicates). f, BS-PCR-seq showing CG, CHG and CHH DNA methylation levels at FWA promoter regions in Col-0, fwa rdr6 and four T1 transgenic lines of dCas9-MQ1v, TRBIP1-dCas9-MQ1v. Pink vertical boxes indicate ZF binding sites. g, Genome browser view showing H3K4me3 ChIP-seq signals at the FWA region in fwa rdr6, dCas9-MQ1v and TRBIP1-dCas9-MQ1v transgenic lines. The numbers in parentheses indicate the data range of the ChIP-seq signals (RPKM) in b and g. Data are presented as mean values ± s.e.m. in a, d and e.
