Extended Data Fig. 2: SunTag-SDG2cd exhibited higher FWA mRNA levels and dCas9 protein expression level in the rdr6 background compared to the Col-0 background. | Nature Plants

Extended Data Fig. 2: SunTag-SDG2cd exhibited higher FWA mRNA levels and dCas9 protein expression level in the rdr6 background compared to the Col-0 background.

From: Histone H3 lysine 4 methylation recruits DNA demethylases to enforce gene expression in Arabidopsis

Extended Data Fig. 2

a, Histogram showing the relative mRNA level of FWA in Col-0, fwa, rdr6, as well as T1 transgenic lines of SunTag-SDG2cd in Col-0 (n = 20 biological replicates) and rdr6 (n = 12 biological replicates) background, respectively. Data are presented as mean values ± s.e.m. b, Western blotting results representing the protein expression levels of HA-dCas9 in both the Col-0 background and T1 transgenic lines of SunTag-SDG2cd in the Col-0 and rdr6 mutant backgrounds, respectively. c-d, The dot plots showing the leaf numbers of Col-0 and SunTag-SDG2cd transgenic lines in the Col-0 background at T1 (c) and T2 (d) generations, respectively.

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