Extended Data Fig. 1: skr mutant alleles show male sterility.

(a) Gene diagram of SKR showing location of insertions: skr-1 (GT_5_101780), skr-2 (CSHL_GT5061), skr-3 (SALKSeq_034497.1). (b) Toluidine blue stained tetrads. The experiment was repeated twice. (c) Inflorescence stem and Alexander staining showing recessive male sterility of skr-1. Mean and S.D. of viable pollen counts per anther (7–12 anthers from each of 4 plants) for WT = 400 +/−51; skr-1/+ = 395 +/−39; skr-1 = 0.9+/−2.1 (d) Complementation of skr-3 by a gSKR transgene. Scale bar: (b) 20 µm; (c, d) 1 cm for inflorescence, 100 µm for anthers.