Extended Data Fig. 9: ITC analysis of the interaction between CDF1Dof and 40-bp DNA fragments containing two tandem repeats of cis elements connected via a 10-base linker.
The upper panel for each experiment displays the representative ITC titration curves (differential power, DP), while the lower panel shows integrated heats (enthalpy, ΔH) of injection (black squares). Each dataset was fitted using the MicroCal origin software with “one binding site” model. The dissociation constant (KD) and the binding molar ratio (N) are listed at the bottom of each set of ITC data, representing the mean ± s.d. from three independent experiments. The DNA sequence highlights the cis element (CTTT) and its mutationally dysfunctional sequence (TCGT), colored in orange and blue, respectively.
