Extended Data Fig. 10: TaCSP-H1 expressions and photosynthetic efficiency of the diploid, tetraploid ancestors and hexaploid wheat.
From: Horizontally acquired CSP genes contribute to wheat adaptation and improvement
a. Venn diagram of photosynthesis-related DEG numbers in diploid Aegilops (Ae. tauschii, 2n = 14, DD) vs synthetic hexaploid wheat (2n = 6x = 42, AABBDD), tetraploid wheat (T. durum, 2n = 4x = 28, AABB) vs synthetic hexaploid wheat, and WT vs TaCSP-H1-OE comparisons. The synthetic hexaploid wheat was generated by hybridizing diploid Aegilops with tetraploid wheat, followed by spontaneously doubling at F1 generation. b. Heatmap of the photosynthesis-related genes in WT and TaCSP-H1-OE in (a). Cluster I, photosystem related genes; Cluster II, electron transport chain related genes; Cluster III, Rubisco related genes. Numbers in the scale bar stand for z-scores of gene expression. c. Expression levels of CSP-H1 homoeologs in diploid Ae. tauschii (DD1, DD2 and DD3), tetraploid wheat (AABB), and three independent lines of synthetic hexaploid wheat (SHW1, SHW2 and SHW3). Each bar shows mean ± SEM of three biological replicates. Different letters represent significant differences (p < 0.05, one-way ANOVA, Tukey’s HSD test).
