Extended Data Fig. 1: Full K_D measurements of substrates.

K_D of substrate binding by PIN8 determined by SSM electrophysiology. Relative inhibition of Peak current response elicited by 100 µM NPA by [substrate] indicated. A Michaelis–Menten model is fit to describe kinetics; data points represent mean ± s.e.m. a) IAA (\({r}_{{IAA}}^{2}\) = 0.91, \({K}_{D}^{{IAA}}\) 40 ± 15 μM; n = 3 individual sensors) b) 2,4,5-T (\({r}_{2,4,5-T}^{2}\) = 0.98, \({K}_{D}^{2,4,5-T}\) 9 ± 0.2 μM; n = 3 individual sensors) c) 2,4-DPP (\({r}_{\mathrm{2,4}-{DPP}}^{2}\) = 0.93, \({K}_{D}^{\mathrm{2,4}-{DPP}}\) 21 ± 3 μM; n = 3 individual sensors). d) 2,4-D (\({r}_{\mathrm{2,4}D}^{2}\) = 0.99, \({K}_{D}^{\mathrm{2,4}D}\) 59 ± 7 μM; n = 3 individual sensors). e) 2-CPA (\({r}_{2-{CPA}}^{2}\) = 0.89, \({K}_{D}^{2-{CPA}}\) 106 ± 5 μM; n = 3 individual sensors) f) MCPA (\({r}_{{MCPA}}^{2}\) = 0.99, \({K}_{D}^{{MCPA}}\) 137 ± 30 μM; n = 3 individual sensors) g) 2,4-DMPA (\({r}_{\mathrm{2,4}-{DMPA}}^{2}\) = 0.99, \({K}_{D}^{\mathrm{2,4}-{DMPA}}\) 174 ± 33 μM; n = 3 individual sensors) h) 2-MPA (\({r}_{2-{MPA}}^{2}\) = 0.99, \({K}_{D}^{2-{MPA}}\) 250 ± 38 μM; n = 3 individual sensors) i) 4-CPA (\({r}_{4-{CPA}}^{2}\) = 0.99, \({K}_{D}^{4-{CPA}}\) 304 ± 37 μM; n = 3 individual sensors) j) PA (\({r}_{{PA}}^{2}\) = 0.95, \({K}_{D}^{{PA}}\) 489 ± 114 μM; n = 3 individual sensors) k) 4-MPA (\({r}_{4-{MPA}}^{2}\) = 0.99, \({K}_{D}^{4-{MPA}}\) 21 ± 3 μM; n = 3 individual sensors).

Source data