Fig. 3: Somatic and heritable editing in Arabidopsis using TRV to deliver ISYmu1 TnpB and guide RNA targeting AtCHLl1.
From: Viral delivery of an RNA-guided genome editor for transgene-free germline editing in Arabidopsis
a, Representative pictures of plants displaying yellow sectors ~2 weeks after TRV delivery. The gRNA and somatic editing efficiency is indicated in the upper left corner of each picture. b, Barplot displaying the somatic editing efficiencies (±s.e.m.) for ISYmu1 gRNA4, gRNA6 and gRNA9 in WT. The gRNA target site is plotted along the X axis. The Y axis indicates the editing efficiencies (percent indel reads). Each dot represents an individual plant that underwent agroflood TRV delivery. c, Representative image of yellow and green progeny seedlings from a WT plant showing 67.4% somatic editing using the TRV2 Architecture_B design with gRNA4 that underwent heat-shock treatment. d, Table summarizing the transmission of edited alleles from four and one individual plants that underwent agroflood delivery using ISYmu1 gRNA4 and gRNA6, respectively. e, Representative Sanger sequencing trace file screenshots from a yellow plant harbouring an edit at gRNA4 (top) or gRNA6 (bottom). For each panel: top, wild-type sequence; middle, the ISYmu1 gRNA target and PAM; bottom, the ab1 trace file displaying a homozygous deletion.
