Extended Data Fig. 1: Schematic of bacterial interference assay plasmids containing either TnpB-ωRNA Single or TnpB-ωRNA Single-HDV design.
From: Viral delivery of an RNA-guided genome editor for transgene-free germline editing in Arabidopsis
The blue arrow indicates the TnpB sequence; the yellow arrow indicates the ωRNA sequence; the black rectangle indicates the guide sequence; the green arrow indicates the HDV ribozyme sequence. The plasmids contain the tetracycline resistance gene (TetR). A tetracycline promoter (pTet) was used to drive expression of the TnpB-ωRNA single or Single-HDV sequences, and the tetracycline resistance gene.
