Extended Data Fig. 6: Amino acid feeding phenotype of the RE1 mutant and metabolic changes upon Lys feeding.
From: RETICULATA1 is a plastid-localized basic amino acid transporter
(a) Representative pictures of 3-week-old Col-0 and re-6 plants grown on ½ MS supplemented with 1 mM proteinogenic amino acids. (b) Representative pictures of 4-week-old Col-0 and re-6 plants grown on ½ MS supplemented with different Arg concentrations. Representative pictures of 3-week-old Col-0 and re-6 plants grown on ½ MS supplemented with different (c) Citr, (d) Lys, and (e) Orn concentrations. Scale bar = 1 cm. The experiments were independently repeated at least three times, with consistent results. Relative (f) Lys and (g) aminoadipate level per mg fresh weight (FW) of 14-d old seedling with and without 1 mM Lys treatment for 2 d. Samples were harvested in the middle of the light period. Data are shown as mean ± SD of four biological replicates. Different letters indicate statistically significant differences between means (P < 0.05; two-way ANOVA). Exact P values (f, g) are shown in source data with 95% confidence intervals.
