Extended Data Fig. 8: De novo amino acid biosynthesis changes in the wildtype and the re-6 mutant after Lys treatment.
From: RETICULATA1 is a plastid-localized basic amino acid transporter
Percent of unlabeled and labeled (a) Ile, (b) Thr, and (c) Met. Metabolites were extracted from the green tissue of 14-day old seedlings, two days after labeling with 4 mM 15NH4Cl with (orange triangles) or without (black circles) 1 mM Lys. Data are shown as four biological replicates. Asterisks indicate statistically significant differences (ns, not significant; *P < 0.05, **P < 0.01, ***P < 0.001; two-sided Student’s t-test). Exact P values (a–c) are shown in source data with 95% confidence intervals. (d) Metabolic changes of Col-0 and re-6 after Lys treatment. Data are shown as log2 fold change of Lys treatment versus control conditions. Schematic depiction of the impact of Lys treatment on de novo Thr, Ile, and Met biosynthesis in the (e) wildtype and (f) the re-6 mutant. The reduced biosynthesis of Thr and Ile in the wildtype due to a feedback inhibition in their biosynthetic pathway (red line) caused by an accumulation of Lys (red arrow) in the plastids by excess Lys treatment (orange triangles) is emphasized by a reduced line thickness. In the re-6 mutant, Lys cannot be efficiently transported into the plastids due to a knockout of RE1 (white circle with red cross).
