Extended Data Fig. 2: Phenotypes of OEX and expression analysis of RE1 and RER1.
From: RETICULATA1 is a plastid-localized basic amino acid transporter
(a) Representative pictures of 5-week-old Col-0, re-6 and complementation lines expressing the coding sequence of RE1 (RE1CDS) or the genomic DNA of RE1 (RE1gDNA) with or without C-terminal GFP fusion in Col-0 or the re-6 mutant background. Scale bar = 2 cm. (b) Representative pictures of 5-week-old Col-0, re-6, re-6 OEX1 and re-6 OEX2 plants (from left to right). Scale bar = 2 cm. (c) Relative RE1 transcript levels in 14-d-old Col-0, re-6 OEX1 and re-6 OEX2 seedlings. Data are shown as mean ± SD of three biological replicates. (d) Representative pictures of 7-week-old Col-0, re-6, re-6 OEX RER1 plants (from left to right). Scale bar = 2 cm. (e) Relative RER1 transcript levels in 14-d-old Col-0, re-6, re-6 RER1 OEX1, re-6 RER1 OEX2 and re-6 RER1 OEX3 seedlings. Data are shown as mean ± SD of three biological replicates. Relative RE1 (grey bars) and RER1 (white bars) transcript levels in (f) 14-d-old seedlings and (g) 34-d-old Col-0, re-6, rer1-1, rer1-2, rer1-3, re-6 OEX1 and re-6 OEX2 rosette leaves. Data are shown as mean ± SD of three biological replicates. (h) Transcript changes of RE1 in Col-0, re-6, rer1-1, rer1-2, rer1-3, re-6 OEX1 and re-6 OEX2 shoots versus root tissue. Data are shown as log2 fold change of shoot versus root tissue.
