Extended Data Fig. 3: Verification of CRISPR/Cas9 dependent editing of RER1.
From: RETICULATA1 is a plastid-localized basic amino acid transporter
Verification of CRISPR/Cas9 dependent editing event in the RER1 sequence of (a) Col-0, (b) re-6 x rer1 (-/+) plant 22 and (c) re-6 x rer1 (-/+) plant 23. Guide sequence is underlined in red. Inference of CRISPR Edits (ICE) analysis of (d) re-6 x rer1 (-/+) plant 22 and (e) re-6 x rer1 (-/+) plant 2362. (f) Growth phenotype of adult Col-0, re-6, and re-6 x rer1 (-/+) plant 22 and 23 (from left to right). Scale bar = 2 cm. Representative pictures of mature siliques of (g) Col-0, (h) re-6, (i) re-6 x rer1 (-/+) plant 22, and (j) re-6 x rer1 (-/+) plant 23. Scale bar = 1 mm. Mature siliques were harvested from three independent Col-0 and re-6 plants, and one re-6 x rer1 (-/+) plant each. Localization of (k) RE1 and (l) RER1-GFP fusion constructs (green) with chlorophyll autofluorescence (magenta) stably expressed in Arabidopsis. Whole seedlings were imaged 15 days after germination Scale bar = 5 µm. The experiment was independently repeated at least three times, with consistent results63,64,65,66,67,68,69,70.
