Extended Data Fig. 9: AT1G19410 (ANH) reduces fertility under heat stress.

(a) Schematic representation of ANH genomic region with positions of the T-DNA insertions. (b-f) Box plots show relative fitness (b), fecundity (c), fertility (d), survival to fruit (e), and shoot dry weight (f) of anh mutants (two independent alleles) relative to Col-0 under heat stress (red) or joint heat and drought stress (purple). Numbers of independent experiments are indicated for relative fitness (b), fecundity (c), and survival to fruit (e). Numbers of plants are indicated for fertility (d) and shoot weight (f). Box boundaries indicate the 25th and 75th percentiles, whiskers extend to 1.5 times the interquartile range, center lines correspond to medians. Different letters signify P < 0.05, one-way ANOVA, Tukey’s test. (g-i) Association of ANH teM with relative fitness (g), survival to fruit (h), and fecundity (i). Correlation coefficients (R) and P values of Pearson’s correlation analysis are indicated.