Extended Data Fig. 10: Root phenotype after PLT2 expression and expression pattern of RBR-YFP in lateral roots.
From: Stem cell regulators drive a G1 duration gradient during plant root development
a, Effect of constitutive PLT2 expression on cell division. Whole root meristems of 6 day old plants expressing PLT2-GR in wild type (wt) or krp5-1 mutant background in the presence or absence of Dexamethasone (Dex) during 30 h. Details of the epidermal layer of each case (white rectangles) are shown in Fig. 3f. Scale bar = 20 µm. b, Quantification of RBR in lateral root primordia (LRP). LRPs of plants expressing RBR-YFP were classified depending on their size in early (0-90 µm) and late ( ≥ 90 µm) stage of development. For quantification, LRPs of seedlings expressing RBR-YFP were divided into two halves: the distal half (solid blue circles), towards the apex of the LRPs and the proximal half (empty circles), towards the base of LRPs. Data points correspond to RBR-YFP signal intensity of individual cells and the median is represented. The number of cells recorded are indicated for each LRP size class below each data distribution inside the graph. The number of LRPs scored for each size class was: 0-90 (n = 8), >90 (n = 5) from 3 roots. Statistical significance was determined using Mann-Whiney Rank Sum tests (two sided): **, p < 0.01; ****, p < 0.0001. Representative images of the lateral root primordia analyzed at different development stages are shown below the graph. Scale bar = 40 µm.
