Extended Data Fig. 7: The endomembrane integrity of rak4m is not different from that of wild type in the root cells.

a–c, Transmission electron microscope (TEM) analysis of root meristem cells displayed similar endomembrane structure of 4-day-old wild type (WT) and ram4m mutants. However, ram4m mutants have enlarged vacuoles in the root meristem cells in contrast to wild type. Representative TEM images of root meristem cells in wild type (a), rak4m-1 (b) and rak4m-2 (c). d–f, Magnified view of the boxed area in wild type (a), rak4m-1 (b) and rak4m-2 (c), with multiple ER and Golgi bodies. PM denotes plasma membrane, ER denotes Endoplasmic reticulum, Golgi indicates Golgi body. Scale bars = 5 μm in a to c, and 500 nm in d to f.