Extended Data Fig. 7: Development of the transient gene expression system in maize leaves.
a, Schematic representation of maize at the V2 stage (left); fluorescence signals of the reporter gene LUC in different maize leaves after transient expression (middle); and statistical analysis of fluorescence intensity (right). L1, L2, and L3 represent the first, second, and third leaves in maize at the V2 stage, respectively. Data are shown mean ± SEM. (n = 3 biologically independent samples). Letters indicate significantly different groups, as determined by one-way ANOVA followed by Tukey’s test at α = 0.05. b, The transcriptional abundance of maize transient expression vectors in L1 leaves was analyzed using two pairs of primers via agarose gel electrophoresis. A schematic diagram of the primer design is shown (top), and PCR products were analyzed on a 2% agarose gel (bottom). ZmActin was used as an internal reference. PCR was performed for 30 cycles with primers 1 + 2, 32 cycles with primers 3 + 4, and 30 cycles with ZmActin. c, Confocal microscopy analysis of fluorescence signals in maize leaves transiently expressing GFP, ZmLecRK1-GFP, and ZmLecRK1N341Q-GFP vectors. Bar = 40 μm. These experiments were repeated 3 times with similar results.
