Extended Data Fig. 4: FgLPMO9A induces the degradation of ZmLecRK1 through the autophagy pathway. | Nature Plants

Extended Data Fig. 4: FgLPMO9A induces the degradation of ZmLecRK1 through the autophagy pathway.

From: An apoplastic fungal effector disrupts N-glycosylation of ZmLecRK1, inducing its degradation to suppress disease resistance in maize

Extended Data Fig. 4

a, Western blot analysis shows that the protease inhibitor Epi1 does not affect FgLPMO9A-mediated degradation of the ZmLecRK1 in N, benthamiana. b, The protease inhibitor Epi1 does not affect the ability of FgLPMO9A to suppress ZmLecRK1-mediated cell death in N. benthamiana. c, FgLPMO9A induces degradation of the full-length (FL) ZmLecRK1 as well as the ECD+TM, but not the intracellular kinase domain (KD) of ZmLecRK1, as shown by the Western-blot in N. benthamiana leaves. d, MG132 and CPZ treatment do not inhibit FgLPMO9A-mediated degradation of the ZmLecRK1, as shown by the western blot in N. benthamiana. e, Effect of different inhibitors treatment on the suppression of ZmLecRK1-mediated cell death in N. benthamiana by FgLPMO9A. All experiment were repeated 3 times with similar results.

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