Fig. 4

Validation of RIM15 as an aging gene by a non-complementation approach. Survival curves and survival integrals were determined using an outgrowth kinetics assay for the indicated hybrid strains in SC medium containing 2% glucose (a) and 0.5% glucose (calorie restriction) (b). The results correspond to mean ± SDs for three biological replicates. Double asterisk, significantly different from control strain by Student’s t-test, p-value < 0.005