Fig. 3
From: Blocking negative effects of senescence in human skin fibroblasts with a plant extract
1201 induces a reticular to papillary transition in reticular HDFs. a Reticular HDFs were cultivated for three PDs in growth medium supplemented with 1201. Representative pictures from one experiment after cultivation for two PDs at ×100 magnification. Scale bar, 100 µm. Subsequent to the treatment as described in a, cells were used for b proliferation assay, c RT-qPCR of markers for the papillary/reticular phenotype (expression levels of untreated control cells were set to 1) and d SA-β-gal staining. For b–d, data represent the average of three experiments. Statistical significance for the treatment in b was calculated with a two-way ANOVA (p = 0.038) followed by a Bonferroni post hoc test
