Fig. 4

1201 attenuates the senescent phenotype in SIPS HDFs. a SIPS was induced by chronic oxidative stress and HDFs were subsequently cultivated for 4 days in growth medium supplemented with 1201. RT-qPCR of p21 (expression levels of untreated quiescent control cells (Q) were set to 1). b SA-β-gal staining of SIPS HDFs after 4, 11 and 18 days of cultivation in growth medium supplemented with 1201. Cells were treated as described in a and RNA-Seq was performed. c PCA analysis and hierarchical clustering. d Venn diagrams identifying the genes which are differential expressed in SIPS compared to Q and are regulated contrariwise by treatment of SIPS with 1201. The pie chart illustrates the identified SASP factors and their response to the treatment with 1201. e Transcript levels of all upregulated SASP factors which were regulated contrariwise by treatment with 1201, displayed as Fragments Per Kilobase of transcript per Million mapped reads (FPKM). Error bars indicate confidence intervals (95%). f Six genes were quantified with RT-qPCR from the same RNA samples which were used for RNA-Seq and correlated with the transcript levels derived from RNA-Seq. From a to f, data represent the average of three experiments. Statistical significance for the treatments in a (p < 0.001 for SIPS treatment and p = 0.088 for 1201 treatment) and b (p < 0.001) were calculated with a two-way ANOVA followed by a Bonferroni post hoc test. The p-values of e were corrected with the false discovery rate for multiple comparisons using the Benjamini–Hochberg method