Fig. 6
From: Blocking negative effects of senescence in human skin fibroblasts with a plant extract
1201 blocks the effects of SIPS HDFs on other cells. SASP-attenuating properties of 1201 were evaluated by pre-treatment of SIPS HDFs with 1201 for 4 days and analysing the growth stimulation of HaCaT cells by either a counting K14-positive cells after co-culture with SIPS HDFs or b determining the cell number after cultivation with conditioned medium. c Representative pictures from one experiment as described in a. Scale bar, 100 µm. d Migration assay using SIPS HDF-derived conditioned medium and human PBMCs. Migratory paths are coloured in black if cells are migrating upwards, in the direction of the conditioned medium. The blue dot marks the centre of mass. e Full-thickness HSEs with SIPS HDFs were constructed as described in the Methods section and the epidermal thickness of control HSEs was set to 1. f Representative pictures from one experiment as described in e. Scale bar, 50 µm. For a, b and e, data represent the average of three experiments. For d, data represent one experiment
