Fig. 2

Light in the blue spectrum is responsible for the decrease in fly lifespan. Lifespan of w a and CS b flies is dramatically reduced in B:D compared with D:D (Log-rank test, p < 0.0001), but minimally reduced in flies aged under white LEDs lacking blue wavelengths by means of a yellow filter (W–B:D). Median lifespan of w c and CS d flies in B:D is reduced with increasing photon flux density (PFD). Statistics shown are from pairwise comparisons of the corresponding mortality curves that showed a dose-dependent effect, increasing PFD from 4 to 11, from 11 to 17, and from 17 to 24 µmol each, causing a significant increase in mortality (Log-rank tests with Bonferroni multiple correction, p < 0.0001). Note that the y axis does not start at 0 to highlight these differences in median lifespan. e Survival of w and CS males kept in B:D or in B:D with added orange light (B + O:D). f Mortality curves of the white-eyed ninaE⁸ and red-eyed ninaE⁷ mutants in B:D and D:D. In all of the above experiments, N = 100 for each genotype and light condition