Fig. 6

Flies maintained in blue light show induction of stress-response genes by day 35. a Expression levels of the indicated stress-response genes in heads of 5- and 35-day-old w males maintained in B:D or D:D. For each gene measured, values from qPCR are reported as fold change relative to expression in young flies in D:D set as 1. Statistics by 2-way ANOVA (****p < 0.0001; **p < 0.01; *p < 0.05). Bars show the average of two biorepeats; error bars show SEM. b Median lifespan of w flies exposed to B:D and/or D:D for the durations indicated by blue or black bars. Flies kept in B:D for the first 30 days of adulthood and then transferred to D:D had a similar median survival to those kept in B:D throughout their entire life, while flies kept in B:D for the first 25 days of adulthood were able to survive much longer (Log-rank test, p < 0.0001). Flies kept in D:D for the first 30 days of adulthood experienced an increased mortality rate upon transfer to B:D. Mortality curves for each condition are shown on the right