Fig. 1: Effect of global Keap1 deletion and diet composition on markers of health in mice.

a Diagram representing the experimental protocol. b Body weight trajectories over the course of 22 weeks after tamoxifen (TAM) administration. n = 8–10 per group. c Lean-to-fat ratio calculation for mice on regular chow and on a western diet. n = 6–8 per group. d Mice were placed into metabolic cages for 48 h to measure the respiratory exchange ratio (RER). n = 6–7 per group. e Left panels, trajectories of blood glucose clearance during an oral glucose tolerance test as percent change from baseline; right panels, area under the curve (AUC). n = 5–10 per group. f Left panels, trajectories of blood glucose clearance during an insulin tolerance test as percent change from baseline; right panels, area under the curve (AUC). n = 6–10 per group. g H&E staining of fixed liver tissues. h Western blot for NQO1 in skeletal muscle extracts. Molecular mass markers (kDa) are depicted on the left. i Densitometric quantification after normalization with Ponceau S staining of the membrane. n = 6–8 per group. All data are expressed as means ± SEM. Comparison by two-tailed t-test was performed unless otherwise specified. *, ***, ****p < 0.05, 0.001, and 0.0001 vs. fl/fl littermate controls.