Fig. 3: Age-associated immune landscape.

a Twenty-eight immune marker genes were identified with our selected genes of the 4 patterns. Immune marker genes were generated from Macaca mulatta single cell sequencing by previous study. TC-WGCNA patterns indicates genes of selected 4 patterns. TC patterns represents genes of all 7 patterns from time-series analysis. b Upset plot of immune marker genes, 7 DEG patterns genes and 4 selected patterns. Colored dots are detailed information of 28 immune marker genes at a. c Scatter plots of immune marker gene patterns while aging for Macaca fasciculairs from our analyzed data and Macaca mulatta from Cayo Santiago population. Colored dots are indication of genes in each 4 selected patterns. Z-scaled normalized value was analyzed in the patternization. Immune type cells shown here are B cell, CD14 + -monocyte, Cytotoxic T cell, Granulocyte, Helper T cell and NK cell. d, e Immune composition level was examined with TIMER 2.0. TPM values were input at the web version. The result excel data was visualized with customized python code. d is the result from TIMER algorithm and e is from CIBERSORT_ABS algorithm for more specific immune cell status. B cell, CD4 + T cell, CD8 + T cell, Neutrophil, Macrophage and Myeloid dentritic cell were shown. Naïve, regulatory and memory types of T cell and B cell are shown. Source data are provided as a Source Data Fig. 3 file.