Fig. 1: The cdkn1a (p21) and 2a (p16) are differentially regulated in aging and HI.
From: p53 dependence of senescence markers p21v1 and p21v2 in aging and acute injury
a–e mRNA levels of p21, p21 variant 1, p21 variant 2, p16 and p53 in the liver, lung, heart and kidney of male mice aged 3 months (3M, young) and 22–24 months (24M, aged), and male mice subjected to hemorrhagic shock injury (HI, young mice). Seven animals per group and at least two technical replicates used. The data was normalized to the geometric mean of three housekeeping genes (HKG): β-actin, β-glucuronidase and Rplp0 (ribosomal protein large P0). The graphs are shown as individual data points along with mean ± SEM. The Y-axes are in log2 scales. Kruskal–Wallis non-parametric test was used. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not-significant.
