Fig. 3: Systemic targeting of p16-expressing senescent cells distinctly modulates age-associated changes in fimbria OPC populations.
From: Aging and senescent fates of oligodendrocyte precursor cells in the mouse brain
a, b High-magnification PDGFRα (green), IBA1 (red), and DAPI (blue) immunostaining images of OPCs in the old (a’, b’) and AP-treated (a’’, b’’) fimbria. Plots of mean values of morphological parameters (c) and mean number of PDGFRα+ OPCs per square millimeter (d) of the fimbria in aged (n = 5) and AP-treated mice (n = 5). e Cumulative distribution of OPC distances to the nearest IBA1+ cell in the old (gray squares) and AP-treated (red triangles) fimbria. f–g High-magnification PDGFRα (green), IBA1 (red), and DAPI (gray or blue) immunostaining images of OPCs in the aged (f’, g’) and AP-treated (f’’, g’’) DG. Plots of mean values of the indicated morphological parameters (h) and mean number of PDGFRa+ OPCs per square millimeter (i) of the DG in old (n = 5) and AP-treated (n = 4) mice. Welch’s t-test was used to compare OPCs from old and AP-treated mouse brains in each region. j Cumulative distribution of OPC distances to the nearest IBA1+ cell in the old (gray squares) and AP-treated (red triangles) DG. Kolmogorov–Smirnov test was used to compare cumulative distributions of the populations (KSD). For cumulative distribution plots, lines on points represent SEM and the inset show a histogram of the same data with a fitted Gaussian curve (old = dotted, AP = solid). ROIs were isolated as described in Methods. All mice represented here are females. Bars represent mean ± SEM.
