Fig. 3: In vivo mouse model of doxorubicin-induced senescence.

A Representative p16 immunofluorescent micrographs from lung of mice receiving vehicle (high oleic acid sunflower oil), 10 mg/kg GinA or 27 mg/kg of modGinA daily for 10 days prior to and 20 days after 10 mg/kg doxorubicin i.p. administration. The negative control group did not receive doxorubicin i.p. administration. Immunofluorescence analysis of colocalized signals for TdTomato (red; p-16) and nuclei stained with DAPI (blue). B Scatterplot of relative tdTomato staining over DAPI between groups (vehicle, GinA, modGinA) and different tissues (one-way ANOVA). Log base 10 scale on relative units. Significant differences between groups are indicated by *0.01 <p < 0.05; **0.001 <p < 0.01 (Mann–Whitney U-test).