Fig. 4: Increased cellular senescence during cardiac aging in the GRZ killifish.
From: Nothobranchius furzeri: a vertebrate model for studying cardiac aging and cellular senescence
a, b Representative images and quantification of senescence-associated beta-galactosidase (SA-β-gal) activity in heart sections from 8 to 16 weeks of age. c–f Representative images and quantification of immunofluorescence staining of γ-H2A.X (a DNA damage marker) co-stained with the sarcomere marker α-Actinin and p15/p16 (a cellular senescence marker) co-stained with the cardiomyocyte nucleus marker Mef2 in heart sections. Scale bars in (a), 100 μm; in (c and e), 20 μm. n = 3–4 animals. One-way ANOVA, Tukey post hoc, *p < 0.05, **p < 0.01.
