Fig. 7: D + Q senolytic treatment removes senescent cells and inhibits SASP marker expression in GRZ killifish hearts.
From: Nothobranchius furzeri: a vertebrate model for studying cardiac aging and cellular senescence
a, b Representative images and quantification of SA-β-gal activity staining in the D + Q treated hearts compared to DMSO controls at 20 weeks of age. Scale bar, 100 μm. n = 3, student’s t test. c–f Representative images of γ-H2A.X and p15/p16 antibody immunofluorescent staining and quantification of γ-H2A.X-positive and p15/p16-positive (arrows) cell numbers in the D + Q treated killifish hearts compared to DMSO. Scale bars, 20 μm. n = 3, student’s t test. g–i Quantitative RT-PCR analysis of cellular senescence marker p21 and SASP markers including tnf-α and il-6 in the D + Q treated killifish hearts compared to DMSO control. n = 3–4 biological replicates, student’s t test. *p < 0.05, **p < 0.01.
