Fig. 2: IgG glycosylation-based biological age - GlycAge - changes significantly in response to the CR intervention.

A Chronological age compared to IgG-based biological age (GlycAge), with the slope (m) of each time interval (0–12mo and 12–24mo) inset, which were different (p = 0.009). However, a multiple comparison test (Tukey’s) comparing the two ages indicated that at 24mo the two ages showed a difference of moderate significance (p = 0.06). B GlycAge for all participants in study – each line represents each participant, connecting dots measured at BL, 12mo, and 24mo. C Biological age (GlycAge) percent change from baseline with significant p-value inset. Differences were evaluated using a mixed model using visit month as a repeated measure, participant as a random effect, sex, and baseline GlycAge as covariates, followed by FDR correction. If the FDR corrected p-values were significant, multiple comparison tests (Tukey’s) were conducted. Tukey’s tests indicated that the 12mo–24mo ages were different (p = 0.027). D, E Change from baseline visit to 12mo and 12–24mo of IgG-based biological age GlycAge and change in body weight. Bivariate regression plots are shown with the β-estimate and corresponding p-value, and Spearman’s correlation coefficient and corresponding p-values, inset evaluating the relationship between change in body weight and GlycAge by time interval (BL-12mo and 12–24mo).