Fig. 4: Effect of 24mo CR on plasma N-glycome evaluated by mixed model tests using visit month as repeated measure, participant as random effect, baseline parameters, sex, and age as covariates, followed by FDR correction.

If the FDR corrected p-values were significant, multiple comparison tests (Tukey’s) were conducted, and significant p-values, if any, were highlighted in red. All figures show percent change from baseline. A, B Non-sialylated glycans were higher, whereas tetra-sialylated glycan species were lower at 24mo compared to BL. C, D Non-galactosylated species increased significantly, while tetra-galactosylated glycans reduced in the 12–24mo period of CR. E High-mannose glycans were significantly higher at 24mo compared to 12mo, but not compared to BL. F Glycans, with bisecting GlcNAc on plasma proteins, were higher at 12mo and 24mo compared to BL. G High-branched glycans were, however, lower at 24mo compared to BL.