Fig. 5: Complement C3 changes due to intervention.

Complement C3 concentration (A) and abundance of distinct C3 glycopeptides B–D glycosylated at asparagine (Asn)-85, and E–G glycosylated at Asn-939) before and after the 2y CR protocol in CALERIE2. All box plots show concentrations of total protein C3 and relative abundance of C3 glycopeptides at baseline, 12mo and 24mo of the CALERIE study in n = 26 participants. Data were evaluated using mixed models with visit month as a repeated measure, participant as a random effect, baseline values, sex, and age as covariates, followed by FDR correction. If the FDR corrected p-values were significant, multiple comparison tests (Tukey’s) were conducted, and significant p-values, if any, were highlighted in red.