Fig. 6
From: Restructuring of Enterococcus faecalis biofilm architecture in response to antibiotic-induced stress
Deletion of epaOX affects lectin-binding ability and polysaccharide composition. a Immunofluorescence micrographs of parent and ΔepaOX biofilms. E. faecalis cells were cultured on Aclar membranes in CBRs for 24 h and stained with the DNA label Hoechst 33342 (blue) and the fluorescein-labeled lectin concanavalin A (ConA—green). Representative images at a magnification of 100× are shown from experiments performed in triplicate. b Carbohydrate composition of purified polysaccharides obtained from parent, ΔepaOX, and ΔepaOX::epaOX strains. E. faecalis strains were cultured to exponential phase for polysaccharide extraction. Error bars represent standard deviation (n = 3 for parent, ΔepaOX; n = 1 for ΔepaOX::epaOX)
