Fig. 2
From: Citrullination mediated by PPAD constrains biofilm formation in P. gingivalis strain 381
Δ8820 biofilms are comprised of more bacterial cells and protein than 381 biofilms. a 381 and Δ8820 were grown for 24 h on glass, stained with SYTO 9 (cells) and SYPRO Ruby (extracellular protein), and images were acquired by CLSM. Images are a top-down view of stacked maximum intensity projection images. Scale bar: 20 µm. b SYTO 9, c SYPRO Ruby, and d total biomass were quantified using Comstat2. Data are averages of two replicates (n = 2). Error bars represent the standard deviation. The data were analyzed using the Student’s two-tailed t-test. *p < 0.05
