Fig. 3 | npj Biofilms and Microbiomes

Fig. 3

From: Citrullination mediated by PPAD constrains biofilm formation in P. gingivalis strain 381

Fig. 3

Δ8820 cells produce a surface-associated extracellular substance that is not present on 381 cells. Bacterial cells from a 381 and b, c Δ8820 colony biofilms grown on plates were negatively stained with 0.5% aqueous uranyl acetate and then imaged by TEM. a 381 displayed typical cell morphology. Scale bar: (left) 2 µm and (right) 200 nm. b Δ8820 cells were (left) surrounded by and (right) coated with an extracellular substance that could be stained by uranyl acetate. Scale bar: (left) 2 µm and (right) 200 nm. c The extracellular substance in Δ8820 samples was capable of (left) forming a meshwork around cells and (right) appeared to extrude from specific sites at the cell surface. Scale bar: 200 nm

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