Fig. 6
From: Citrullination mediated by PPAD constrains biofilm formation in P. gingivalis strain 381
Δ8820 biofilms contain more gingipain-derived adhesin proteins than 381 biofilms. a Western blot analysis of biofilm lysates and planktonic cell lysates using an anti-adhesin primary antibody. ΔrgpA and Δkgp planktonic cell lysates were used to determine the specific adhesin proteins (from top to bottom: Rgp44, Kgp39, Rgp27). Mfa1 was used as a loading control. All identified bands are shown. Samples derive from the same experiment and the blots were processed in parallel. b, c Quantification of b biofilm and c planktonic blots shown in a. ND, none detected d Suspended 381 and Δ8820 colony biofilms were treated with the anti-adhesin primary antibody and then images were acquired by immunogold labeling and SEM. Gingipain-derived adhesin proteins localized to the surface of 381 cells, the surface of Δ8820 cells, and the matrix around Δ8820 cells. Arrows indicate representative colloidal gold particles. SE secondary electron, BSE ackscatter electron. Scale bar: 2 µm
