Fig. 7
From: Citrullination mediated by PPAD constrains biofilm formation in P. gingivalis strain 381
Deletion of PPAD decreases secreted Rgp enzymatic activity and increases Rgp and Kgp enzymatic activity in biofilms. Rgp enzymatic activity in a planktonic cultures and c biofilm lysates, and Kgp enzymatic activity in b planktonic culture and d biofilm lysates was measured using a colorimetric assay. a Less Rgp activity was measured in the supernatants of Δ8820 cultures. b There were no differences in Kgp activity between 381 and Δ8820. Both c Rgp and d Kgp enzymatic activities were greater in Δ8820 biofilm lysates than 381 biofilm lysates. Data are representative of three independent experiments showing similar results (n = 3). Error bars represent the standard deviation of technical replicates. The data were analyzed using the Student’s two-tailed t-test. *p < 0.05
