Fig. 2 | npj Biofilms and Microbiomes

Fig. 2

From: Primer-free FISH probes from metagenomics/metatranscriptomics data permit the study of uncharacterised taxa in complex microbial communities

Fig. 2

Effectiveness of cell sorting and enrichment level of cells that have been processed with FISH–FACS. a Cell sorting purity obtained after an initial round of sorting was calculated from flow cytometric analysis. b Quantitative FISH analysis depicting the relative abundance of target cells in pre-sorted and sorted samples. Quantitative FISH analysis was performed by acquiring confocal images, followed by biovolume image analysis. Each dot represents quantitative FISH analysis performed on one confocal microscope image. c Relative abundance of ribotags matching the R-Probe and OTU in metagenomic DNA of pre- and post-sorted samples. Each dot represents a sequenced sample. d Comparison of the environmental specificity of probes Thau646 and Ribo_Thau1029_17. The sorting was performed in various event cut-offs and the legend denotes the different number of sorted events

Back to article page