Fig. 4 | npj Biofilms and Microbiomes

Fig. 4

From: Primer-free FISH probes from metagenomics/metatranscriptomics data permit the study of uncharacterised taxa in complex microbial communities

Fig. 4

Phylogenetic analysis of marker genes retrieved from UPWRP_1. a Maximum-likelihood (PhyML) phylogenetic tree depicting the 16S rRNA gene phylogenetic relationship of UPWRP_1 and its closely related sequences in the SILVA 123 SSU Ref NR99 database. Members of the genus Chlorobium were used as the outgroup. Only near full-length sequences (≥1200 bp) were selected. Representative sequences of OTUs are demarcated in red, and sequences of the closest cultured isolates are demarcated in blue. Bootstrap values were calculated from 1000 bootstrap analyses, and only bootstrap values over 50% were displayed. Branches with low bootstrap values ≤50% have been multifurcated. The scale bar represents substitutions per nucleotide base. Legend of the bootstrap values is located at the upper left-hand corner of the diagram. b Phylogenetic placement of genomics bin of UPWRP_1 in the reference genome tree using a concatenation of 43 phylogenetic marker genes. A set of 2502 complete genomes and 3604 draft genomes from IMG database were used in the reference genome tree. Genomic bin of UPWRP_1 is demarcated in red. Closest outgroup corresponded to different classes under the phylum Bacteroidetes

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